IMAGE

Fig. 3

ID
ZDB-IMAGE-151030-4
Genes
Source
Figures for Antonio et al., 2015
Image
Figure Caption

Fig. 3

Wounding leads to increased proliferation of pre-neoplastic cells.

A–D′ RasG12VeGFP larvae were left unwounded (A–D) or laserwounded (yellow dotted line) at 2 dpf just dorsal to the cloaca (A′–D′). Larvae were left to grow for 3 days before being fixed and analysed for preneoplastic cell number (n = 30 larvae in each group). EdU accumulation (purple) is shown at low magnification (C and C′) and a representative preneoplastic cell clone is shown (D and D′).

E. Graph illustrating preneoplastic cell numbers in unwounded versus wounded larvae (n = 27 of each group). Data from three independent experiments showed the same level of significance. ***P d 0.001.

F–G′ Images of larvae injected with control morpholino at the onecell stage, and either left unwounded (F, high magnification: G) or laserwounded at 3 dpf (F′, high magnification: G′) and fixed at 5 dpf.

H–J′ Larvae injected with a combination of PU1 and GCSF morpholinos at the onecell stage, left unwounded (H, high magnification: I) or laserwounded at 3 dpf (H′, high magnification: I′) and fixed at 5 dpf. Images of larvae injected with irf8 morpholino, and either unwounded (J) or laserwounded at 3 dpf (J′) before subsequent fixation at 5 dpf.

K, K′ Larvae injected with GCSF morpholino, and either left unwounded (K) or laserwounded at 3 dpf (K′) and fixed at 5 dpf.

L, M Graphs to show the total number of Ras+ cells and clones (respectively) at 5 dpf after PU1 and GCSF morpholinos.

N, O Graphs showing the number of Ras+ cells at 5 dpf after irf8 morpholino (N) or after GCSF morpholino injection (O).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO J.