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Fig. S2
Hemiray thickness increases during fin growth; RA signaling is not required for osteoblast survival.
(A) Sections of uninjured fins of different fin lengths reveal correlation between fin length and hemiray thickness. Fin lengths: 8, 4.5 and 2.5 mm. Yellow bars: Hemiray thickness. (B) ISH demonstrates expression of aldh1a2 in proximity to hemirays (arrowhead). (C) Inhibition of RA signaling does not interfere with survival of mature osteoblasts. IHC for ZNS-5 demonstrates a similar number of osteoblasts in wild type and hsp70I:cyp26a1 fish upon 10 days of heat-shock treatment. (D and E) ISH demonstrates expression of cyp26a1 (D) and cyp26c1 (E) in single epidermal (arrow) and mesenchymal (arrowhead) cells in uninjured fins. Scale bars: 20 µm in A, C; 50 µm in B, D and E. hs, heat-shock.
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