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Fig. 2
Stump osteoblasts upregulate cyp26b1 expression. (A) Fin amputation induces upregulation of aldh1a2 and cyp26b1 expression. qPCR analysis. P<0.05 unless noted otherwise. (B) IHC for ZNS-5 and GFP in aldh1a2:gfp fish at 24hpa reveals aldh1a2 expression in fibroblasts (arrowhead). (C) WISH indicates upregulated cyp26b1 expression within one segment length proximal to the amputation plane at 18hpa. Longitudinal section on WISH shows upregulated cyp26b1 expression in single osteoblasts (arrowhead). Scale bars: 20µm in B and C (section); 50µm in C WISH. Dashed lines indicate amputation planes.
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