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Fig. S4
Overexpression of Yap S87A in vsx2 positive cells results in ectopic expression of dct mRNA and -2.7kb tfec:Gal4-VP16/dsRed:UAS:Yap S87A positive cells do not migrate into the neural retina.
(A) Endogenous dct expression is observed in the RPE. (B-B′′) Ectopic dct expression is observed in the neural retina and enhanced in the ciliary marginal zone in Yap S87A overexpressing embryos. Black arrows denote ectopic dct. (C-C′′′) RPE cells overexpressing Yap S87A do not ectopically migrate into the neural retina. Images represent a 2 hour time course from 40-42 hpf. White arrows indicate a Yap S87A positive RPE cell.
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