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Fig. S3
qRT-PCR validates mRNA transcripts that were upregulated via RNAseq in Yap S87A overexpressing 36 hpf eyes and -1.0kb ctgfa:d2GFP is upregulated by Yap S87A.
(A) ctgfa (9.5-fold, p=0.0508), cyr61 (12.6-fold, p=0.1072), clu (135.5-fold, p=0.1705), fn1b (195-fold, p=0.2418), hbegfa (2.4 –fold, p=0.0845), lats2 (6.7-fold, p=0.0005), pawrl (17.6-fold, p=0.1736). Dashed line indicates the normalized expression levels of yap and taz in wild-type embryos. An unpaired t-test was performed on mRNA expression and statistical significance was performed using the Holm-Sidak method. Error bars = s.e.m. (B-B′) -1.0kb ctgfa:d2GFP expression is observed in the NR, RPE, heart, and other tissues at 28 hpf. (C-F′′) Yap S87A overexpression results in increased -1.0kb ctgfa:d2GFP in RPE cells (F-F′′) and ectopic NR expression (E-E′′) compared to mCherry (C-D′′). Arrows=ectopic NR, arrow heads=RPE expression. All embryos are 28 hpf.
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