ZFIN Image: Miesfeld et al., 2015, Fig. 2

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Figure Caption

Fig. 2

yap^nl13/nl13 mutants exhibit coloboma. (A) Splice site variants elicited by the yap^nl13 allele. The mutation results in aberrant splicing and an early stop codon in the transactivation domain for all described products. (B) Protein schematics for wild-type and predicted mutant yap^nl13 allele variants. (C) Western blot showing the loss of full-length Yap in yapⁿ^l^13/nl13 mutants. A smaller protein product is detectable at higher levels in the mutant (~40 kDa). (D-D′′,G-G′′) 3dpf wild-type and yap^nl13/nl13 embryos showing coloboma (arrows) in the absence of other overt phenotypes. Plastic sections of wild-type (D′′) and mutant (G′′) eyes show the coloboma (arrow) phenotype and RPE deficits that are sometimes observed in the ventral retina. (E,F,H,I) Sections showing Yap and Taz proteins (green) in wild-type and yapⁿ^l^13/nl13 mutant eyes. Red counterstain (TO-PRO3) shows nuclei.

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Phenotype details

Acknowledgments

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