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Fig. S4
Eight hours of 33°C treatment is sufficient to alter aPKC localization and reduce shha expression in sde1 regenerates.
(A) Antibody co-staining for aPKC (red) and P63 (green) in longitudinal sections of sde1/+ and sde1 fin regenerates after 8 hours of 33°C treatment at 3 dpa, indicating rapid loss of basal cell polarity. Scale bars, 50 µm. (B) shha RNA expression is also reduced in sde1 regenerates after 8 hours of 33°C treatment (n = 5 each). Scale bars, 100 µm.
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