ZFIN Image: Wilson et al., 2015, Fig. 1

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Figure Caption

Fig. 1

Manipulation of glucocorticoid activity induces changes in cardiac structure Confocal images of embryonic (120 h post fertilization (hpf)) of Tg (CMLC2: GFP) zebrafish hearts after 120 h glucocorticoid receptor (GR) manipulation with either GR agonist dexamethasone, (Dex) [100 µM] or targeted GR knockdown using morpholino (GR Mo). Whole mounted GFP embryonic hearts (green) were stained with DAPI (blue) and imaged at high power with a confocal microscope. Gross cardiac morphology can be observed at ×20 magnification, higher power images (×40 magnification) demonstrate advanced maturity of the Dex treated hearts in contrast to the GR Mo hearts which show considerable immaturity of myofibrillar and trabecular patterning. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

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Reprinted from Molecular and Cellular Endocrinology, 414, Wilson, K.S., Baily, J., Tucker, C.S., Matrone, G., Vass, S., Moran, C., Chapman, K.E., Mullins, J.J., Kenyon, C., Hadoke, P.W., Denvir, M.A., Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart, 120-31, Copyright (2015) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.