ZFIN Image: Hou et al., 2015, Fig. 3

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Figure Caption

Fig. 3

Induced androgenetic doubled haploids and clonal line in zebrafish (Danio rerio).

(a–f) The four different types of larvae hatched from eggs subjected to cold-shock treatment for 30 min at 7 ± 0.5 °C, followed by heat-shock treatment at 43 min AF. (a) and (b) Normal wild-type larva; (c) abnormal wild-type larva; (d) and (e) normal golden-type larva; and (f) abnormal golden-type larva. Scale bar denotes 1 mm. (g) DNA fingerprints were determined by amplified fragment length polymorphism using the selective primer combination E-AGC/M-CTT. dl: DNA ladder; B: golden-type male doubled haploid whose sperm was used for androgenesis induction; c1–c7: normal golden-type diploid progeny hatched from androgenesis (clonal fish); i1–i4: female intact control; i5–i8: male intact control.

Acknowledgments

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