Fig. S5

Fig. S5 EGFP expression in the VZ of un-injected (UN) Tg(ptf1a:EGFP) embryos, or embryos of the same line injected with the indicated klf8-MOs between 35 and 48 hpf. (A-L) Dorsal views of embryos. (A-D) At 35 hpf, EGFP expression is detected in the LRL of UN embryos (A), and embryos injected with klf8-4mmMO1 (B) or klf8-MO1^atg (C) or co-injected with klf8-MO^AC and klf8-MO^DO (D). (E-L) EGFP expression is also detected in the VZ of UN embryos (E, I) and embryos injected with klf8-4mmMO1 (F, J) at both 40 and 48 hpf. However, EGFP is not detected in the VZ of embryos injected with klf8-MO1^atg (G, K, arrowhead) or co-injected with klf8-MO^AC and klf8-MO^DO (H, L, arrowhead) at either 40 or 48 hpf. LRL, lower rhombic lip; VZ, ventricular zone.