IMAGE

Fig. 2

ID
ZDB-IMAGE-151012-3
Source
Figures for Swinburne et al., 2015
Image
Figure Caption

Fig. 2

α-bungarotoxin immobilizes embryos while permitting normal development.

(A) Percent of embryos immobile after injection of α-bungarotoxin protein (0.046–4.6ng) into the yolk at 24 hpf. (B) Percent of embryos immobile after injection of α-bungarotoxin mRNA (20–400 pg) into the yolk at 24 hpf. (C) Percent of embryos immobile after injection of of α-bungarotoxin mRNA (5–100 pg) into the 1-cell zygote. (D) Percent control OVD at 72 hpf for injection of α-bungarotoxin mRNA into the 1-cell zygote (green), into the yolk (yellow), and reference anesthetic treatments that permitted long-term immobilization (blue). (*) Not significantly different from control, Mann-Whitney-Wilcoxon two tailed P-value 0.87. (†) Significantly different from control, Mann-Whitney-Wilcoxon two tailed P-value 0.0011. (E, G) Control embryo at 72 hpf that was injected with 50 pg of membrane-citrine mRNA into the 1-cell zygote. (F, H) 72 hpf embryo that was injected with 50 pg of α-bungarotoxin mRNA into the 1-cell zygote. (I) Control larva at 8 days post fertilization (dpf) injected with 50 pg of membrane-citrine mRNA into the 1-cell zygote. (J) 8 dpf larva that was injected with 50 pg of α-bungarotoxin mRNA into the 1-cell zygote.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One