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Fig. S1
dchs expression in zebrafish
A. Whole-mount in situ hybridization of dchs2 in WT embryos at 4-cell (1 hpf), shield (6 hpf), 90% epiboly (9 hpf), and 24 hpf stages.
B. Quantitative RT-PCR analysis of dchs2 expression levels in MZdchs2stl1/stl1 mutants relative to WT embryos at maternal and zygotic stages.
C. Relative expression by qRT-PCR of dchs1b in WT and MZdchs1bstl1/stl1 embryos.
D. Relative expression by qRT-PCR of dchs1a in WT, MZdchs1bfh275/fh275, and MZdchs1bstl1/stl1 embryos.
E. Relative expression by qRT-PCR of dchs2 in WT and MZdchs1bfh275/fh275 embryos.
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