Fig. S11

Fig. S11 Percentage of apoptotic HSPC in kri1l^cas002 mutants and WT siblings. Representative confocal images of kri1l^cas002 mutants and WT siblings after TUNEL assays co-staining with EGFP antibody (in Tg(cmyb:egfp) background) in different time points and regions, including the AGM at 40 hpf (A-H) (A and E are bright-field images overlaid with fluorescent staining. Scale bars, 25 µm.); the CHT at 40 hpf (I-P) (I and M are bright-field images overlaid with fluorescent staining. Scale bars, 25 µm.), at 2 dpf (Q-X) (Q and U are bright-field images overlaid with fluorescent staining. Scale bars, 25 µm.), and at 3 dpf (Y-F′) (Y and C′ are bright-field images overlaid with fluorescent staining. Scale bars, 25 µm.). (G′) Statistic analysis of the apoptotic HSPC percentage (the proportion of apoptotic HSPC in total HSPCs) in kri1l^cas002 mutants and wild-type siblings during hematopoietic development (40 hpf-3 dpf). Ne10 for each group. 40 hpf AGM, p=0.6824; 40 hpf CHT, p=0.5971; 2 dpf CHT, p=0.6433; 3dpf CHT, p=0.9122. Error bars represent standard error of the mean (SEM). **, P≤0.01; ***, P≤0.001 (Student t test).