ZFIN Image: Rydeen et al., 2015, Fig. 3

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Figure Caption

Fig. 3

Embryonic RA is decreased in VP-RAR expressing embryos. (A, B) vp-rar mRNA injected into Tg(β-actin:GDBD-RLBD);(UAS:EGFP) embryos shows significant loss of GFP expression indicating embryonic RA is reduced compared to WT. 25% (n=7 of 28) of control embryos from the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) outcrossed to a WT adult showed strong RA sensor expression, while 22% (n=8 of 37) of vp-rar mRNA injected embryos had dramatically reduced RA sensor expression. (C,D) RA levels are greatly diminished after heat-shock induction of the egfp-vp-rar transgene in Tg(β-actin:GDBD-RLBD);(UAS:mCherry) embryos compared to siblings without the heat-shock inducible transgene.

Acknowledgments

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Reprinted from Developmental Biology, 405(1), Rydeen, A., Voisin, N., D'Aniello, E., Ravisankar, P., Devignes, C.S., Waxman, J.S., Excessive feedback of Cyp26a1 promotes cell non-autonomous loss of retinoic acid signaling, 47-55, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.