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Fig. S10
plk2b regulates zebrafish endothelial cell migration and sprouting in vivo via Rap1 activity. (A) Injecting 100 pg of ca-RAP1 into (A) plk2b MO Tg(kdrl:mcherry-ras) fish could partially rescue the plk2b MO vascular defects (n=42/127). (B) Additionally, injecting 160 pg of ca-RAP1 into plk2b MO Tg(kdrl:mcherry-ras) fish could not only partially rescue the plk2b MO vascular defects but also occasionally lead to ectopic EC branching (yellow arrowheads) (n=41/78). (C) Injecting 80 pg of ca-RAP1 into wild-type Tg(kdrl:mcherry-ras) fish also resulted in occasional ectopic EC branching (yellow arrowheads) (n=57/127). Scale bar, 80 µm. Top, dorsal longitudinal anastomotic vessel; bottom, dorsal aorta/cardinal vein. (D) Quantitative measurements of the number of ISV branches show more ISV branches in 80 pg ca-RAP1 injected embryos and in plk2b MO1 embryos rescued with 160 pg ca-RAP1 when compared to control and plk2b MO1 embryos rescued with 100 pg ca-RAP1. Mean±s.e.m. *p<0.05 by ANOVA.
Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.