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Fig. 4 Interaction between retinoblastoma cells and vasculatures
(a) A representative image of the entire fli1:EGFP zebrafish embryo that received SJmRBL-8 retinoblastoma cell implantation at day 4. The left eye was implanted with DiI-labeled tumor cells (red) and zebrafish vasculatures were visualized by expression of EGFP. Arrows point to the metastatic tumor cells in the head and trunk regions. Bar = 100 µm. (b) Detection of DiI-labeled metastatic retinoblastoma cells in the contralateral eye by confocal imaging analysis. (c) Visualization of implanted retinoblastoma cells in relation to hyaloid vasculatures at different points after tumor cell implantation. Arrows indicate the position of primary tumors growing in tight association with hyaloid vessels. Bar = 100 µm.