ZFIN Image: Wei et al., 2015, Fig. S5

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Figure Caption

Fig. S5

NER-independent role of CSB in repair of strand breaks at a transcribed region. (A) Effects of siRNA for the indicated protein for HR and NHEJ. (B) HeLa and XP12RO (XPA^-/-) cells were pretreated with or without DRB (20 γM) for 24 h, then irradiated with IR at the indicated dose. Colony forming assays were performed, and the survival fraction is shown.(C) U2OS TRE cells with FBS starvation were treated with or without the indicated siRNA, then transfected with TA-KR. Cells were irradiated with light for 10 min followed by 30 min/48 h of incubation, then fixed and immunostained with anti-γH2AX and anti-53BP1. The percentage of γH2AX and 53BP1 foci positive cells (defined as the fold increase of foci at TA-KR sites compared with background intensity being >2) was quantified. (D) α-amanitin (100 µg/mL) for 1 h, DRB (20 µM) for 24 h, or siCSB pretreated cells were fixed, followed by immunostaining with anti-AcH3K9.

Acknowledgments

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