Fig. 4
Nfatc1 expression within the endocardium is lost in the absence of myocardial cells.
In situ hybridization analysis for endocardial nfatc1 expression (A-D) and myocardial myl7 expression (E-H) in control 1% DMSO-treated embryos (left column), control myl7:mCherry-NTR-negative embryos treated with 10mM MTZ (middle column) and myl7:mCherry-NTR+ embryos treated with 10mM MTZ (right column). Loss of myocardial myl7 expression is observed in G,H (right column, arrows), which correlates with the loss of nfatc1 expression (C,D; right column, arrows). (A,B,E,F) Flat-mount embryos, ventral view; (C,D,G,H) whole-mount embryos, ventral view of the heart region.