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Fig. S7 NA Differentiation and VMC Coverage in Mouse and Quail Embryos, related to Figure 7. (A-D) Transverse sections of quail (A) and mouse (B-D) embryos immunostained with antibodies against SMA (A-C, red), NG2 (D, orange) and TH (A-D, green). Note close proximity of TH+ SNs (arrows) and SMA-covered regions of the DA (B), but presence of TH+ aggregates in SMA-negative regions of the DA (C) in more posterior sections of the same embryo. Further analysis of consecutive serial sections shows these areas are already covered with NG2+ immature VMCs (D). DA; dorsal aorta; Scale bar: 50 µm in A-D.
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