ZFIN Image: Iyengar et al., 2015, Fig. 1

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Figure Caption

Fig. 1

Melanocyte Regeneration in Adult Zebrafish Requires mitfa-Expressing Cells

(A) Strategy for identifying cells that mediate melanocyte regeneration: promoter-based ablation of only differentiated melanocytes results in regeneration (left) whereas promoter-based ablation of differentiated melanocytes and cells that mediate melanocyte regeneration causes failure of regeneration (right).

(B) Adult zebrafish expressing mitfa:NTR in miniCoopR-rescued melanocytes and lineally related cells (top). Differentiated melanocytes were ablated upon Mtz treatment (middle) but failed to regenerate after Mtz was washed out (bottom). n = 5 fish; representative images are shown. mC, miniCoopR. Scale bar, 5 mm.

(C) Adult p53(lf) zebrafish expressing miniCoopR-mitfa:NTR (top). Differentiated melanocytes were ablated on Mtz treatment (middle), and new melanocytes regenerated after Mtz was washed out (bottom). n = 5 fish; representative images are shown. mC, miniCoopR. Scale bar, 5 mm.

See also Figure S1 and Movies S1 and S2.

Acknowledgments

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Reprinted from Developmental Cell, 33(6), Iyengar, S., Kasheta, M., Ceol, C.J., Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells, 631-43, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell