IMAGE

Fig. 1

ID

ZDB-IMAGE-150827-2

Genes

Citrine

Publication

Ruf-Zamojski et al., 2015 - Spatio-Temporal Differences in Dystrophin Dynamics at mRNA and Protein Levels Revealed by a Novel FlipTrap Line

All Figures

Figures for Ruf-Zamojski et al., 2015

Image

Figure Caption

Fig. 1 FlipTrap Gt(dmd-citrine)^ct90a line allows visualization of Dmd protein.

(a). Schematic of the FlipTrap vector inserted within dmd locus. Citrine insertion (green) occurs within intron 4–5 in dmd in the Gt(dmd-citrine)^ct90a trap line (top) and upon translation produces a fluorescently tagged full-length functional Dmd protein (bottom). The exons (blue rectangles), introns (blank line) and domains of the protein are not drawn to scale. (b-e). Wide-field fluorescent images of Dmd-Citrine expression in the Gt(dmd-citrine)^ct90a line, in the trunk skeletal muscles (b-c) at 24hpf (b) and 32hpf (c); (d-e). Expression in the cranial skeletal muscle at 6dpf (lateral (d) and ventral (e) views). Ventral view showing expression in the skeletal muscle of the branchial arches. Scale bars: (b)-(c) 100µm, (d)-(e) 25µm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One