ZFIN Image: Helker et al., 2015, Fig. 1, S1

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Figure Caption

Fig. 1, S1 Inhibition of Vegfa signaling resulted in normal angioblast migration to the midline, but impaired sprouting angiogenesis.

(A–J) Vegfa signaling was impaired using either ligand MOs (vegfaa/vegfab MO), chemical inhibition of Vegfr2 signaling (2.5 µM SLKB1002 [Zhang et al., 2011]) or mutations in the vegfr2 gene (kdrl). Confocal projections of Tg(fli1a:EGFP)^y1 embryos in dorsal views at 17 hpf (A, B, C, G, H, I), 24 hpf (F, J, K, L) and 28 hpf (D, E). At 17 hpf, angioblasts in control embryos as well as Vegfa signaling deficient embryos arrived at the midline (A, B, C, G, H, I). Control embryos showed sprouting of the intersegmental vessels (D, F, K) whereas Vegfa signaling deficient embryos lacked the intersegmental vessels (E, J, I).

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