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Fig. S2 (A-C) her2-MO inhibited EGFP expression driven by the her2 5′-UTR. (A) her2 5′UTR sequence used for driving EGFP expression, where the start code ATG of her2 is labeled as “red”, and the her2-MO sequences are underlined. (B, C) the her2 5′UTR-EGFP reporter expression was detected in control embryos (B), but not in her2-MO morphants (C). (D-F) Id1-MO inhibited EGFP expression driven by the id1 5′-UTR. (D) id1 5′UTR sequence used for driving EGFP expression, where the start code ATG of her2 is labeled as “red”, and the her2-MO sequences are underlined. (E, F) the id1 5′UTR-EGFP reporter expression was detected in control embryos (E), but not in id1-MO morphants (F). (G-M) Knockdown of mecp2 increases neural precursors and astrocytes while suppressing neuronal development but either her2 or id1 knockdown has the opposite effects. RNA in situ hybridization with nestin, gfap, map2, and neurod probes in wild-type (WT) embryos (G-G′′′), embryos injected with mecp2-mis MO (H-H′′′), mecp2 morphants (I-I′′′), embryos injected with her2-mis MO (J-J′′′), her2 morphants (K-K′′′), embryos injected with id1-mis MO (L-L′′′), and id1 morphants (M-M′′′) at 48 hpf. Note that the expression of nestin and gfap were up-regulated while map2 and neurod were down-regulated in mecp2 morphants; and the expression of nestin and gfap were down-regulated while map2 and neurod were up-regulated in both her2 morphants and id1 morphants. Dorsal views with anterior to the left. The numbers in the bottom-right corner represent phenotypic embryos/total embryos.