Fig. 1, S2 Generation of gpr124 mutant zebrafish.
(A) Maximal intensity projection of a confocal z-stack of Tg(kdrl:GFP) wild-type and gpr124s984/s984 cranial vasculature at 5 dpf in dorsal views color-coded by depth. Dorsal most vessels are blue, ventral most vessels are red. Scale bar, 100 µm. (B) DIC images (left panel) and maximal intensity projection of a confocal z-stack of Tg(kdrl:GFP) wild-type and gpr124s984/s984 cranial vasculature at 14 dpf in dorsal views color-coded by depth (right panels). Dorsal most vessels are blue, ventral most vessels are red. Scale bar, 100 µm. (C) Survival curves of wild-type and gpr124s984/s984 mutants. The survival of respectively 34 and 26 animals was recorded.