ZFIN Image: Evason et al., 2015, Fig. S7

Image

Figure Caption

Fig. S7 SP600125 and amitriptyline do not significantly affect fabp10a promoter activity or Wnt reporter activity.

(A) Representative photographs of fabp10a:EGFP expression (the same fabp10a promoter element used to drive pt-β-cat expression) in control (top row) and Tg(fabp10a:pt-β-cat) (bottom row) zebrafish livers at 5 dpf. (B) Fluorescence intensity ± standard error of the mean (SEM) was quantified using ImageJ, and samples were compared using 2-way ANOVA; p>0.05 for each group compared to every other group. N values are shown above the x-axis. (C) Representative photographs of 7xTCF-Xla.Siam:GFP (Wnt reporter) expression in control (top row) and Tg(fabp10a:pt-β-cat) (bottom row) zebrafish livers at 6 dpf. (D) Fluorescence intensity ± standard error of the mean (SEM) was quantified using ImageJ, and samples were compared using 2-way ANOVA (p>0.05 for all drug treatments compared to DMSO control of same genotype.) Scale bars, 20 µm. Six zebrafish were analyzed for each group.

Acknowledgments

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