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Fig. 8

ID
ZDB-IMAGE-150716-2
Source
Figures for Kotani et al., 2015
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Figure Caption

Fig. 8

Visualization of endogenous gene expression by knock-in of the eGFP reporter using the crRNA-tracrRNA-Cas9 protein complex.

(A) Whole-mount in situ hybridization with an epdr1 probe. (B) An uninjected embryo. (C) The embryo injected two crRNAs (epdr1-crRNA; 25 pg + Mbait-crRNA; 25 pg), tracrRNA (100 pg) and Mbait-hs-eGFP (25 pg) with Cas9 protein (400 pg). The expression of eGFP was detected in the anterior central nervous system, including neurons.

Acknowledgments
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