ZFIN Image: Wong et al., 2015, Fig. 5

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Fig. 5

Retinal progenitor cells undergo premature cell cycle exit in dmbx1-overexpressing embryos. Dmbx1a and dmbx1b mRNAs were co-injected in the embryos at the 1-cell stage to test whether Dmbx1 can sufficiently promote cell cycle exit in retinal progenitor cells. Indeed, when dmbx1 genes are misexpressed, these embryos (B and D) have reduced amount of proliferating cells in the retina when compared to un-injected embryos (A and C) as indicated by BrdU (A and B) and pHH3 (C–E) staining at 1 dpf. These progenitor cells are forced to become post-mitotic prematurely. Un-injected and misexpressing embryos were assessed for Zn5 (F and G), GS (H and I), and Zpr1 (J and K) at 72 hpf. There was a noticeable expansion of Zn5 cells in the ectopic dmbx1 embryos.

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Acknowledgments

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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.