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Fig. S4
Heat-shock of hemizygous or homozygous Dnfgfr1 transgenics. Transgenic embryos at either 24 hpf or 48 hpf were heat-shocked once for 60 min at 37 °C followed by normal rearing at 28.5 °C until the desired stage. Our analyses suggested that the intensity of GFP expression and the severity of the loss of function phenotype varied according to the developmental stage at which the heat-shock was administered rather than transgene copy number. For this reason, our analyses included mixed populations of hemizygous and homozygous transgenic embryos (including the data shown in the chart).
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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.