ZFIN Image: Wong et al., 2015, Fig. 1

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Fig. 1

Transplantation experiments demonstrated that dmbx1 functions cell autonomously in the developing retina. Coronal sections of 72 hpf host embryos transplanted with β-actin:GFP positive donor cells at blastula stage. Cells from un-injected (UN) or morpholino-injected (MO) Tg(β-actin:GFP) donor embryos were transplanted to either UN- or MO host embryos. Animals with mosaic retinae were selected for further analysis using immunohistochemistry: Pax6 (RGCs and amacrine cells) and Zpr1 (cone photoreceptor) (A–D, n=6 in each UN donor group and n=3 in each MO donor group); and phospho-Histone H3 [pHH3] (E–H, n=6 in each UN donor group and n=3 in each MO donor group). Images were captured using a confocal microscope without any maximum projection. White dotted boxes highlight areas where GFP+ donor cell localization in the host retina. Numbers of pHH3+ cells within those transplanted cells in the retina of the host embryos are summarized in graph (I).

Acknowledgments

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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.