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Fig. 5 Representative confocal micrographs of 24–28 hpf zebrafish embryos, showing chd knockdown phenotypes depending on experimental protocol. (A) Wildtype embryo, uninjected. (B) Ru-MO-chd, incubated in the dark, showing normal development. (C) Ru-MO-chd, irradiated for 5 min at 1 hpf with 450 nm light, showing chd knockdown phenotype. (D) Bis-azido MO-chd showing chd knockdown phenotype. All embryos were injected at 1-cell stage and imaged with a 10× air objective (Olympus UPlanSApo, NA = 0.40). (E) Percentage of embryos showing wildtype or degree of phenotypic response.
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