ZFIN Image: Sato et al., 2015, Fig. S6

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Figure Caption

Fig. S6 Injection of MO^nrg1IIE1 does not induce apoptosis in the optic tectum.

A. Immunohistochemical staining of embryos injected with MO^nrg1IIE1 and the control MO^nrg1IIE15m with anti-activated Caspase-3 antibody at 50 hpf. Higher magnification of the Caspase-3-positive punctum is shown in the inset. Arrow, Caspase-3-positive puncta; dotted circle, optic tectum (OT). B. Quantification of the number of Caspase-3-positive puncta in the OT for the experiment shown in A (mean ± s.e.m.; nrg1IIE15m, 6.2 ± 3.54, n = 5, nrg1IIE1, 5.0 ± 2.05, n = 5, p = 0.78, unpaired t test).

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