ZFIN Image: Rossi et al., 2015, Fig. 1

Image

Figure Caption

Fig. 1

Microglia Derive from ALPM Yolk-Sac Macrophages with No Contribution from the ICM

(A) Photoconversion of ALPM macrophages. Side views of a 22-hpf photoconverted embryo (mpeg1::Kaede) are shown (top left, Kaede green; top right, Kaede red; bottom left, overlay of green and red; bottom right, overlay with bright field).

(B–D) Side view shows a 3-day-post-fertilization embryo previously photoconverted at 22 hpf (B, Kaede green; C, Kaede red; D, overlay).

(E) Photoconversion of ICM macrophages. Side views of a 36-hpf photoconverted embryo (mpeg1::Kaede) are shown (arranged as in A).

(F–H) Side view shows a 3-day post-fertilization embryo previously photoconverted at 36 hpf (F, Kaede green; G, Kaede red; H, overlay). Red labeling in the eye is due to autofluorescence of the sample.

(I–K) Dorsal view shows a 4-day-post-fertilization embryonic brain previously photoconverted at 22 hpf in the ALPM region (I, Kaede green; J, Kaede red; K, overlay). Dotted line indicates the shape of the brain and the two hemispheres.

White arrowheads indicate Kaede⁺ cells showing both green and red emission. Scale bars for all images, 50 µm. See also Figure S1.

Figure Data

Expression / Labeling details

Acknowledgments

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