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Fig. 11
Proliferation patterns in 18-somite stage embryos. I: Left side views of 18-somite embryos. II: Dorsal views of 18-somite embryos. (A, B) Full confocal slice projections. Asterisk indicates the yolk extension. (C, D) Partial confocal slice projections where most superficial slices were omitted to highlight internal patterns. White doted lines highlight different structures like the eye (e), neural tube (nt), and otic vesicle (ov). In D, the arrowhead indicates the border between mesencephalon and rhombencephalon and the arrows mark rhombomeres limits. (E, H) Dorsal view of 18-somite stage embryo. The embryo shows abundant PH3 positive nuclei in the main axis as compared to the yolk cell (H). (G, H) Amplification of corresponding inserts shown in E and F to highlight the dorsal region of the neural tube (white arrowheads), that shows mitotic nuclei arranged in two rows along the antero–posterior length of the embryo. 200 µm scale bar in B. 100 µm scale bar in H.