ZFIN Image: Boer et al., 2015, Fig. 6

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Figure Caption

Fig. 6 Depletion of fscn1a-independent NC cell filopodia by low concentration Latrunculin B treatment does not enhance the severity of fscn1a MZ craniofacial defects.

(A) Lateral views of leading edge of cranial NC stream 1 in 26 hpf DMSO or Lat. B-treated Tg(sox10:rfpmb) or Tg(sox10:rfpmb); fscn1a MZ embryos. Scale bar = 25 µm. (B) Quantitation of filopodia number and filopodia length in DMSO or Lat. B-treated Tg(sox10:rfpmb) and Tg(sox10:rfpmb); fscn1a MZ embryos (n = 9 Tg(sox10:rfpmb) + DMSO, n = 10 Tg(sox10:rfpmb) + Lat. B, n = 10 Tg(sox10:rfpmb); fscn1a MZ + DMSO, n = 10 Tg(sox10:rfpmb); fscn1a MZ + Lat. B, *p = 0.02, **p<0.005, ***p<0.001, ****p<0.0001, ns = not significant). (C) Representative ventral views of 5 dpf DMSO or Lat. B-treated wild type (wt) and fscn1a MZ embryos stained with Alcian blue. (D) Quantitation of percentage of embryos with normal or abnormal craniofacial development at 5 dpf. Numbers above columns indicate number of embryos included in analysis (n = 83 wt + DMSO, 93 wt + Lat. B, n = 86 fscn1a MZ + DMSO, n = 72 fscn1a MZ + Lat. B, *p = 0.02).

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Acknowledgments

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