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Fig. 6
The effect of NO on tumor growth.
CM-Dil labeled glioma cells were injected into the yolk of zebrafish embryos at 2 dpf and incubated with CPTIO (200µM). Images were taken at 1 dpi and 4 dpi. Images (A-A′′′) and (B-B′′′) show the tumors at 1 dpi and 4 dpi for control and CPTIO treated embryo. At 4 dpi, tumors have increased in size in untreated embryos (A′′-A′′′) in comparison to CPTIO treated embryos (B′′-B′′′). (C) Percentage of fluorescence intensity of the tumor surfaces was determined by ZEN software (n = 10). Images were taken with an Axio zoom V16 (Zeiss) macroscope, with the same exposure time for all embryos. Error bars represent SEM, *** p <0, 001, (one way ANOVA). (D) QRT-PCR analysis of Cyclin D1 gene expression in untreated and CPTIO treated xenografted embryos. Cyclin D1 expression was decreased upon CPTIO treatment. One representative experiment from four independent experiments is shown.