IMAGE

Fig. 4

ID
ZDB-IMAGE-150601-125
Source
Figures for Yousfi et al., 2015
Image
Figure Caption

Fig. 4

Alkaline phosphatase assay of xenografted DAF-FM-DA treated embryos.

Embryos were injected with red CM-Dil labeled tumor cells, incubated at 4 dpi in DAF (5µM) and fixed at 4dpi before detection of alkaline phosphatase activity. (A) Bright field image of the embryos before treatments. (B) Neovascularisation was observed in 12 of 14 (85.5%) embryos presenting NO production in tumor environment. (D) Fluorescence detection of glioma cells, (C) DAF signal, (E) merge. The white arrows indicate the part of the tumor mass that co-localizes with DAF fluorescence signal. (F) A control embryo with absence of vessels in this region of the yolk.

Acknowledgments
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