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Fig. 1

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ZDB-IMAGE-150518-36
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Image
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Fig. 1

Surface cell differentiation in zebrafish germ-layer explants and aggregates. (A and A′) Time course of surface cell differentiation in ectoderm explants from Tg(krt4:EGFPcaax)/Tg(βactin:H2AmCherry) transgenic embryos expressing EGFP (green) in differentiating enveloping cell-layer (EVL) cells and mCherry (red) in all nuclei. Z projections of a representative explant at consecutive time points (A) and a single confocal section (A′) through the explant shown at the last time point. (Scale bar: 100 µm.) Time labels indicate minutes after preparation of the explants from high-to-oblong–stage embryos [3 and 5 h postfertilization (hpf)]. (B–D) Immunostaining of ectoderm explants using antibodies against atypical protein kinase C (aPKC) (apical membrane, red) and Zona Occludens 1 (ZO-1) (tight junctions, white). The plasma membrane is labeled with lynEGFP (green). (Scale bars: 25 µm.) (E and E’) Time course of surface cell differentiation in ectoderm aggregates from Tg(krt4:EGFPcaax)/Tg(βactin:H2AmCherry) transgenic embryos expressing EGFP (green) in differentiating EVL cells and mCherry (red) in nuclei. Z projections of a representative aggregate at consecutive time points (B) and confocal section through the aggregate shown at the last time point (B′). (Scale bar: 100 µm.) Time labels indicate minutes after dissociation of high-to-oblong–stage embryos (3 and 5 hpf).

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA