ZFIN Image: Lee et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1

Overexpression of IFT46 induces apoptosis in zebrafish embryos. (A, B) Control and human IFT46 mRNA (100 pg/embryos) injected embryo stained with acridine orange at 24 hpf. overexpression of IFT46 leads to increase of apoptosis in developing embryo. (C) Temporal expression profile of ift46 by RT-PCR. The ift46 transcript is detected from 4-cell stage to 72 hpf in zebrafish. Both maternal and zygotic expression of ift46 is detected. β-actin is a loading control. (D and E) ift46-GFP expression is completely inhibited when co-injection with ift46 translation blocking morpholino in zebrafish embryos, showing the efficacy of the ift46-MO.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 400(2), Lee, M.S., Hwang, K.S., Oh, H.W., Ji-Ae, K., Kim, H.T., Cho, H.S., Lee, J.J., Yeong Ko, J., Choi, J.H., Jeong, Y.M., You, K.H., Kim, J., Park, D.S., Nam, K.H., Aizawa, S., Kiyonari, H., Shioi, G., Park, J.H., Zhou, W., Kim, N.S., Kim, C.H., IFT46 plays an essential role in cilia development, 248-57, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.