|
Fig. S7
Steroidogenic cells display a rise of Vimentin expression which is induced by hemodynamic forces and pFak signaling.
Vimentin in the steroidogenic tissue (marked by green fluorescence) of Tg(ff1bEx2: GFP) embryos was increased from 2 dpf (A-A′) to 2.5 dpf (B-B′). The increase in Vimentin was not observed in (C, C′) tnnt2a morphants, (D, D′) RGD-treated embryos, or (E, E′) klf2a or (F, F′) mmp2 morphants. Sections are shown of a representative embryo from each treatment group. (G) Fluorescence intensity of Vimentin in ff1bGFP-expressing steroidogenic tissue (ROI marked by orange lines) is normalized to the size of the cluster, with the number of embryos indicated in parentheses. The difference between 2-dpf control group and any of the other groups was analyzed by Student′s t-test. **P<0.005, N.S., not significant. D, dorsal; V, ventral; L, left; R, right. Abbreviations: notochord (NC). Scale bar, 25 µm.