ZFIN Image: Glenn et al., 2014, Fig. 4

Image

Figure Caption

Fig. 4

scl functions downstream of etv2 during neutropoiesis in the ALPM. (A-D) scl RNA can rescue anterior mpx expression in etv2 knockdown embryos, as analyzed at 24 hpf. mpx expression in etv2 morphants (B, n=19/19) is decreased as compared to the wild type siblings (A, n=26/26). Injection of scl RNA rescues mpx expression in etv2 morphants (C, n=23/29). All embryos are positioned with anterior to the left and dorsal side up. (E) Morpholino knockdown of scl reduces the induction of mpx by etv2 DNA, as analyzed by real-time RT-PCR at the 12-somite stage. mpx expression is increased in etv2 DNA injected embryos, relative to uninjected controls but is not changed significantly when etv2 DNA is co-injected with scl MO. Relative expression levels have been normalized to EF1α expression. Asterisk denotes statistical significance vs. etv2 DNA over-expression (p<0.01) as analyzed by one-way ANOVA test with pair-wise comparison. Error bars correspond to±standard deviation. (n=3).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 393(1), Glenn, N.O., Schumacher, J.A., Kim, H.J., Zhao, E.J., Skerniskyte, J., Sumanas, S., Distinct regulation of the anterior and posterior myeloperoxidase expression by Etv2 and Gata1 during primitive Granulopoiesis in zebrafish, 149-59, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.