|
Fig. 11
Depolarization-induced regenerative calcium wave in the presence of TTX.
(A) Peak response of the Fluo-4 calcium signal following depolarization from 80 mV to +50 mV for 2 min in the presence of 1 µM TTX. The color coded ROIs are indicated. (B) Measurements of ΔG/R from the ROIs indicated in (A) show that calcium propagation traveled in two successive, distinct movements into the boutons. Each data point represents the flattened stack of 15 sections, each 1 µm thick, acquired with 1 s intervals between stacks. Experiment was performed with 0.5 mM EGTA in the intracellular solution. (C) Time to reach 50% of the first peak was plotted against distance from the soma for the example. ROIs shown in A and B are indicated with colors and additional ROIs not shown are black. The data points were fit to a line. The video for this recording and a second example recording are available as Video 3 and Video 4 respectively.