ZFIN Image: Wang et al., 2014, Fig. 5

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Figure Caption

Fig. 5

PF1052 inhibited neutrophil migration in a PI3K-independent manner. (A) Schematic diagram of the lyz:PHAkt-EGFP construct used to generate the stable transgenic line. (B) Time-lapse imaging of migrating neutrophils showed PHAkt-EGFP signal was always localised to the cell front in control larvae. The red signal indicates the highest level of PI3K at the leading edge. (C) PI3K inhibitor LY294002 reversed the cellular localisation of PI3K, which accumulated within the cell body. (D,E) Extracts from Sphaeropsidales and its functional component PF1052 did not affect the localisation of PI3K at the cell membrane. (F-I) Quantification of neutrophil polarity index in DMSO-, LY294002- and PF1052-treated larvae. LY294002 and PF1052 significantly reduced neutrophil polarity index (***P<0.001).

Acknowledgments

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