ZFIN Image: Wang et al., 2014, Fig. 1

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Figure Caption

Fig. 1

Inhibitory effect of PF1052 and sterigmatocystin on neutrophil migration towards the wound site. Neutrophil migration assay was carried out using Tg(mpx:GFP)ⁱ¹¹⁴ larvae according to Renshaw et al. Renshaw et al., 2006). (A) Neutrophils were quiescent on an uninjured 3-dpf larva. (B) About ten neutrophils were recruited to the wound site 3 hours after the tailfin was amputated on a control larva that was treated with DMSO. (C,D) Larvae treated by PI3K inhibitor LY294002 (50 µM) or microtubule inhibitor nocodazole (33 µM), respectively, had few neutrophils recruited to the wound. (E) An extract (ID: XF06-5B03) from an ascomycete genus Sphaeropsidales completely blocked neutrophil migration towards the wound at 50 µg/ml. (F) The active component identified from XF06-5B03 extract (PF1052) also completely blocked neutrophils recruitment at a very low concentration of 2 µM; the molecular structure of PF1052 is shown in J. (G,H,K) Another extract, XF06-2A10, from a fungus, genus Aspergillus, that blocked the migration of neutrophils contains the functional component sterigmatocystin, working at 50 µM. (I) Quantification of neutrophils recruited to the site of injury (n>15). The white line on B-H indicates the amputation site of tailfin. ***P<0.001.

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