ZFIN Image: Nakayama et al., 2013, Fig. 10

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Figure Caption

Fig. 10

Effects of transcriptionally active and repressive gbx2 on brain formation. (A) Schematic views of constitutively active and repressive Gbx2. The homeodomain of Gbx2 is placed downstream of the EnR domain and VP16 activator domain (VP16-AD)(En-Gbx2 and VP-Gbx2, respectively). (B) Characteristic phenotypes of embryos overexpressing modified gbx2 genes. Embryos were injected with mRNA for egfp (100 pg/embryo), gbx2 (30 pg/embryo), en-gbx2 (30 pg/embryo), or vp-gbx2 (100 pg/embryo) and observed at 26 hpf. (a–d) Lateral views with anterior to the left and dorsal to the top. (a′–d′) Dorsal views with anterior to the left. Normal and disrupted isthmuses are shown with solid and open large triangles, respectively. ey, eye; FB, forebrain; HB, hindbrain; MB, midbrain; ov, otic vesicle. Scale bar, 200 µm.

Acknowledgments

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Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.