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Fig. S6 lhx9 overexpression induces ectopic QRFP neurons. (A-D) Ectopic qrfp:EGFP expressing neurons that persist until 96 hours post HS (120 hpf) also express ectopic lhx9 (arrowheads in C, D). (E-H) Double fluorescent ISH for lhx9 and qrfp in WT 120 hpf larvae shows an absence of lhx9 expression in the hindbrain region that contains lhx9-induced ectopic qrfp neurons in (A-D). Boxed regions in (A, B, E, F) are shown at higher magnification in (C, D, G, H). (A, B, E, F) show 95 µm thick confocal maximum intensity projections containing both endogenous and ectopic QRFP neurons. (C, D, G, H) show 43 µm thick confocal maximum intensity projections including only the region containing ectopic QRFP neurons. lhx9-expressing neurons that appear close to ectopic QRFP neurons in (B) are located 30 µm ventral to the ectopic QRFP neurons, and are thus not observed in (D). Scale = 10 µm.