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Fig. S4 Time course of endogenous hcrt and lhx9 expression. Confocal projections of Tg(hcrt:EGFP) embryos at 24, 48, 72, 96, and 120 hpf show that all Hcrt cells express lhx9 throughout development, as determined by fluorescent ISH for lhx9 followed by anti-GFP immunostaining. Ventral images are shown. Scale = 10 µm.
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