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Fig. S2 Strategy to identify genes enriched in embryonic zebrafish Hcrt neurons. Transgenic zebrafish embryos were dissociated into single cells at 26 hpf and cells of interest were purified by FACS. RNA from purified neurons was amplified and hybridized to microarrays. Signals from Hcrt neurons were compared to other neuron types and genes most highly enriched in Hcrt neurons were identified. Highly enriched transcription factors and secreted peptides were selected for verification by ISH and overexpression analysis in zebrafish.
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