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Figure Caption
Fig. S1 Eph-ephrin gene expression within the 30-hpf zebrafish head. Fluorescent in situ hybridization (green) shows expression of efnb2a (A,B), efnb3b (C,D) and ephb4a (E,F) relative to sox10:GFP+ neural crest-derived mesenchyme (red in A,C,E) and nkx2.5:GFP+ mesoderm (red in B,D,F). In addition to staining in pouches p3, p4 and p5, we also observed expression of efnb2a in neural crest-derived cells (arrowheads in A) and ephb4a expression in ventral arch mesoderm (arrowheads in F) adjacent to more mature pouches. Scale bar: 20 µm.
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