Fig. 6

Fig. 6

Coexpression of MBNL2 suppresses GFP(CUG)₉₁ mRNA-induced phenotypes. (A) Representative images at 24 hpf of an embryo injected with mRNA encoding MBNL2-mCherry. (B) Representative images at 24 hpf of an embryo co-injected with mRNAs encoding MBNL2-mCherry and GFP(CUG)₉₁. (C) Quantification of mbnl2 mRNA by qRT-PCR in GFP mRNA, GFP(CUG)₉₁ mRNA and MBNL2-mCherry-injected embryos at 24 hpf. (D) Blinded quantification of the abnormal head phenotypes in embryos injected with the indicated RNAs. (E) Blinded quantification of the abnormal tail phenotypes in embryos injected with the indicated RNAs. (F) Survival at 24 hpf in embryos injected with the indicated RNAs. (G) Spontaneous coiling behavior at 24 hpf in embryos injected with the indicated RNAs. (H) Touch-evoked swim escape response at 48 hpf in embryos injected with the indicated RNAs. For D-H, more than 100 embryos per group were evaluated in at least three independent experiments. Equal amounts of GFP(CUG)₉₁ mRNA were injected in the presence or absence of mbnl2 mRNA. *P<0.05, **P<0.001 on two-tailed unpaired t-test except for survival data, which was analyzed using a chi-squared test.