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Fig. 7
High sensitivity of otx2 in the anterior neural plate to gbx2 overexpression at the bud stage. (A) Changes in the sensitivity of otx2 in the anterior neural plate to gbx2 overexpression during development. Offspring embryos from crosses between wild-type and hsp70l:gbx2+/– Tg fish were exposed to heat shock (HS) at the stages specified at the left and examined 2 h after HS for otx2 expression. Each panel is composed of a dorsal (left) and lateral view (right), and the positions of the MHB are marked with solid triangles. ‘+’ and ‘’ show intensities of otx2 expression relative to those in normal embryos (+++). The proportions of the defects and total numbers of scored embryos are shown in the respective panels. Scale bar, 200 µm. (B) Genotyping of embryos subjected to heat treatment and in situ hybridization. See the legends to Fig. 4 and Fig. 5 for details. The results show that downregulation of otx2 depends on the presence of hsp70l:gbx2 (arrowhead).
Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.